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James C. M. Hwang

Department of Electronic and Computer Engineering, Lehigh University, Bethlehem, Pennsylvania United States


Contributions

  • Correlation Between Optical Fluorescence and Microwave Transmission During Single-cell Electroporation
    Correlation Between Optical Fluorescence and Microwave Transmission During Single-cell Electroporation

    In this study, multimodal characterization of a mammalian cell by optical and microwave techniques simultaneously during electroporation. Using a coplanar waveguide with a Jurkat cell trapped in the middle of its center conductor, continuous waves at 100 kHz of different amplitudes were applied for 20 s to porate single cells while microwave transmission coefficients at 9 GHz were measured every 0.4 s. The onset of electroporation was indicated by abrupt changes in both fluorescence intensity and transmission coefficient. Experimental results show that the transmission coefficient could serve as a label-free indicator of cell membrane permeability during and after electroporation.

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