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Jianfei Sun

Jianfei Sun is with the School of Biological Sciences and Medical Engineering, Southeast University, Nanjing 210018, China


Contributions

  • Real-time Temperature Measurements of HMEC-1 Cells during Inflammation Production and Repair detected by Wireless Thermometry
    Real-time Temperature Measurements of HMEC-1 Cells during Inflammation Production and Repair detected by Wireless Thermometry

    Cell inflammation and its repair process are extremely important for inflammation treatment. The processes can be reflected through real-time in situ cellular temperature changes. By detecting the HMEC-1 cellular temperature under lipopolysaccharide inflammation production and norepinephrine for inflammation repair, combining the changes in cell viability, inflammatory factor levels and ATP content caused by different lipopolysaccharide or norepinephrine doses, an obvious inflammatory response and repair effect was obtained. Temperature variations were correlated with ATP content. The relationship between cell thermogenesis and intracellular energy reserves is related to cell processes. Analysis of the energy changes in different physiological process can be realized.

  • Wireless thermometry for real-time temperature recording on thousand-cell level

    It is vitally necessary to measure the cellular temperature to fully understand life sciences. A method allowing to measure the cellular temperature for a normal growing state without doing damage to the cells and disturbing their intercellular communication is needed. Here, a wireless, real-time, high-throughput temperature detection method is developed. The acquisition system applies a high-precision reference resistor and a low real-time measurement current (below or equal to 0.14 mA) to reduce self-heating via the intermittent measurement. Cells of a small volume cell medium are cultured on the surface of the platinum thermal resistor and subsequently measured in the incubator.

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